| Carnation Mottle Virus (CarMV) is an important viral agent infecting carnation and causing considerable loss to this crop. The research was conducted to study the effect of extracts of medicinal plants on CarMV. The infected plants were treated with extracts of Asparagus sp., Ocimum spp. and Vitex sp. prepared in different solvents by soxhlet apparatus. Laboratory bio-assay as well as studies under field conditions was done to see their effect on viral diseases. The presence of virus was evaluated using DAS-ELISA and scoring was done through microtitre plate reader at 405 nm wavelength. Asparagus extract in acetone (AA) & methanol (AM) appears to exhibit maximum inhibitory potential against CarMV, when added to the medium after autoclaving at 10 mg/l concentration. There was no loss of inhibitory potential due to heat when this extract was added before autoclaving the medium. Asparagus extract in methanol (AM) was also effective at 10 mg/l. in both cases i.e. addition before and after autoclaving. Extract of Ocimum sanctum in water (OSW) was also found effective; however, its effectiveness was confined only to, after autoclaving, additions of the extract to the medium. Field spray on the other hand could not eliminate the virus under test. However, inhibition to certain degree was recorded. |