| Chromatography is one of the most useful group of techniques available for the separation of closely related compound is preset in a mixture. Here the separation is effected by differences in the equilibrium and distribution of the components between two immiscible phase, namely the stationary and the mobile phases. Thin layer chromatography (TCL) is a technique, easy to perform and requires a simple apparatus. It readily provides qualitative information and it is possible to obtain quantitative data also with careful attention to details. The present papers deals with the application of the TCL technique for qualitative detection of pesticide residues in fish. This technique has been proved to be specific, rapid and sensitive in qualitative determination of pesticide in fish tissues. In the present study TLC was applied to fish treated with carbamate (carbaryl) organochloro (Endosulfan) and organophosphorous (malathion) pesticides. In all studies it has been observed that this technique is a simple easy one, specific and it is possible to detect quantities of pesticides hence, its use in strongly recommended for the detection of these poisonous chemical in fish. |